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Protein
Chemistry Technology Center
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Welcome
to the Protein Chemistry Technology Center at the University of
Texas, Southwestern Medical Center. The core facility was established
to provide efficient, high quality services to researchers in the
academic community.
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Director:
Associate
Director:
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Steve
Sprang, Ph.D
Haydn
Ball, Ph.D
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Peptide
Synthesis:
Currently
we have three automated peptide synthesizers, including two multiple
peptide synthesizers that operate at either 25 µmol, 50 µmol,
0.1 mmol or 0.25 mmol scales. Turnaround time is usually around
2 weeks for crude peptides or 2-3 weeks for purified peptides. Crude
peptides are delivered as dry powders and percentage purity is dependent
on length and amino acid sequence. We guarantee >70% purity for
crude peptides and >95% purity for RP-HPLC purified products.
All peptides are returned with a Mass Spec analysis and an analytical
RP-HPLC chromatogram.
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Charges
and Special Services:
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Scale
(µmol)
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Crude
(>70% purity)
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Purified
(>95% purity)
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25
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$200
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$450
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50
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$300
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$550
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100
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$500
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$750
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250
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$650
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$900
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250
(res 21-51*)
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$45/res
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*
sequences longer than 51 residues TBD.
We offer a wide variety of modifications e.g. phosphorylated Ser,
Thr, Tyr; biotinylation and labeling with fluorescent probes. Please
call with specific needs.
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Protein
Sequencing:
The
ABI 494 Procise Edman sequencer can handle samples at low picomole
levels, either attached to PVDF membrane or in solution. If in solution
please contact lab for acceptable buffers and solvents. Results
are usually returned within a few days.
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Charges
Residues
1-5 $100; additional residues $20 per cycle.
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| Mass
Spectrometry:
Fast
mass determination and purity check is provided using MALDI-TOF-MS
or triple quadrupole ESI-MS instruments. Post-translational modification
analysis can also be performed. Protein identification services
are offered by Dr.
Yingming Zhao's facility.
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Charges
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| Mass
determination: |
$52 |
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| Sample
desalting: |
$32 |
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| de
novo MS/MS analysis: |
$70/hr |
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| Facility
Location: |
South
Campus Y4.332
5323 Harry Hines Blvd
Dallas Tx 75390
Phone: 214-648-5031
FAX: 214-648-9477
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| Contact: |
Peptide
Synthesis: |
Hayden
Ball |
214-648-6769 |
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N-Terminal
Sequencing and Mass Determination: |
Bikash
Pramanik |
214-648-5031 |
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For
General Information: |
Hayden
Ball |
214-648-6769 |
