The Laboratory of Amelia J. Eisch

Diane Lagace (Maiden name: Bird) is the Canadian in our group and joined the lab in August 2004. Diane received her Bachelor of Science degree in honors psychology at McMaster University in Hamilton Ontario in 1996, and then moved to Nova Scotia to work in clinical research in the Department of Psychiatry at Dalhousie University with Dr. Stanley Kutcher. Wanting to understand more about the biological mechanism of action for medications used to treat psychiatric conditions, she pursued graduate work at Dalhousie University in Department of Pharmacology where she obtained her Master of Science degree in 2000 and PhD in 2004. Her thesis work published in 3 refereed papers examined the neuroendocrine effects of valproic acid, a mood stabilizing drug, and demonstrated that valproic acid inhibited adipogenesis.

Combining her background in psychopharmacology with adult neurogenesis, Diane has has several projects up and running in the lab, which have already resulted in 5 publications in the Eisch Lab. Most of current work is focused on characterizing and utilizing the Nestin-CreERT2 inducible transgenic mouse to track and permanently label the newly born cells in the brain. By crossing this mouse with other floxed mouse models, Diane is determining in vivo the functional role for neurogenesis by ablating neurogenesis, as well as examining how specific proteins, such as cyclin dependent kinase 5 (cdk5), has a unique functional role in neurogenesis. Diane also has a keen interest on the connection between neurogenesis and depression and explores this relationship through evaluating how neurogenesis may be involved in animal models of depression and antidepressant efficacy. She is currently funded by a postdoctoral research award from the Canadian Institute of Health Research (CIHR) and has recently applied for a NARSAD Young Investigator Award.

Outside the lab, Diane keeps active by working in her garden and exercising. To offset this physical activity, she enjoys cooking (makes treats for the lab meetings), trying out different Dallas restaurants, or just hanging out at home with her husband, Tom (also a UTSW postdoc) and planning for the expected birth of their son in August 2007. Diane has clarified for us that Canada is not the tundra and enjoys vacations back home to see her family, friends, and nature at its best at the cottage in Muskoka. What a view!

Stephanie Fischer, PhD

Stephanie joined the group in June 2005. She received undergraduate degrees in English Literature and Biology at California State University at Sonoma, where she learned how to make wine in chemistry class, but wasn t old enough to taste it. She received her PhD at Mayo Graduate School in Rochester, Minnesota, and spent three years thawing out at the University of Colorado Health Sciences Center, before joining the Eisch Lab in Texas.

Stephanie is currently working on an injection model of morphine exposure, to see if these mice have alterations in hippocampal cytogenesis, vascular-related growth factors, and learning behavior. Her work was funded by an NIH training grant.

Stephanie finished her fellowship with us at the end of May, 2007, with several manuscripts published or in the works. We're sad she's gone from the lab, but happy she is close by as she starts her new position with Dallas Independent School District as a Science Educator and Neuroscience Liason for hospital-bound and brain-injured DISD school children.

Michael Donovan

Mike joined the group in May 2003. He received his undergraduate degree in Biochemistry and Cell Biology at Rice University - Go Owls! He hails from Austin, but he likes Dallas primarily because of the science at UT Southwestern Medical Center, but also because of "the big hair and the fact that Dallas is not Houston".

Mike has a diversified lab portfolio at this point. He first worked on a mouse model of Alzheimer's Disease, the PDAPP mouse. Mike used a variety of classic and novel approaches to identify distinct alterations in hippocampal cytogenesis and neurogenesis. This is a project in collaboration with the German Laboratory that is generously funded by the Alzheimer's Disease Center, an NIH funded institution. Currently Mike is working on several other projects to investigate how antidepressants actually act to increase adult hippocampal neurogenesis, how a clinically-relevant model of chronic stress alters adult neurogenesis, and the behavioral implications of stem cell ablation in the adult mouse.

Mike's current work is funded by an individual predoctoral fellowship from the National Institute of Mental Health, and he has one major publication and several manuscripts in the works. Rock on, Mike!

When Mike is not in the lab, he enjoys his house (with massive lawn) and his dog Dobes (yes, a Doberman). He also really, really likes hiking in Colorado (and apparently cooking in the rain in Colorado, see picture). On a past trip he had harrowing encounters with a moose (quite dangerous, despite its rounded antlers) and a badger, although he returned with scars more remniscent of a bear attack. Luckily, the last time there were no moose or badger attacks.

Mike is also a welding fanatic, and not surprisingly, Flashdance is his favorite movie. He does NOT like chocolate, and many chocoholics in the group hold this against him. He's got a mean swing with a driver despite the fact that graduate students aren't supposed to have time for golf.

Gwyndolen Harburg

Gwyn joined the group in August 2004. She received her undergraduate degree in Biotechnology at University of California, Davis. She is a former graduate student of Richard Gaynor's (now at Eli Lilly) at the UT Southwestern Comprehensive Cancer Center. She also worked for several years as a research associate in the lab of David Amaral at the UC Davis M.I.N.D. Institute studying the pathology and etiology of autism and William's syndrome.

Gwyn is a proud member of "Team Opiate". Her dissertation project involves examinging how opiates and opioids impact adult hippocampal neurogenesis. She's also characterizing adult neurogenesis in the human hippocampus. She's been anointed the "ImmunoQueen" or "Golden Fingers Harburg" for her gorgeous immunohistochemistry.

Gwyn is finally making a go at finishing her dissertation before it's time to retire, or at least before her June 8, 2007 defense date. Why the rush? Well, Gwyn has been awarded a summer fellowship from the National Science Foundation to study in Australia, and has also secured a fully supported postdoctoral fellowship in Australia (coincidence?) studying mammary stem cells in the lab of Jane Visvader at the Walter and Eliza Hall Institute of Medical Research in Melbourne. G'day Mate!

Amy Arguello

Amy joined the lab in January 2005, after receiving her undergraduate degree in Biology with minors in Music and Chemistry from St. Mary's University. Amy works on addressing the mechanism by which morphine causes a decrease in neurogenesis. Could morphine alter the length of the cell cycle or alter levels of growth factors which could subsequently lead to a decrease in neurogenesis? These are just a few of the questions that interest Amy. Amy is supported by a grant from the National Institute of Drug Abuse.

Another side project that Amy works on, but of equal importance, is maintenance of the Emergency Word of the Week (aka Emergency Phrase or Warning of the Week). Very important in instances of extreme emotion, for example: "Yowzers Francine! That gel looks great!" or "Yowzers, (long pause) Francine, that gel didn't work".

Michele Noonan

Michele joined the lab in January of 2005, after completing a summer rotation in 2004. She received her Bachelor's degree at Boston College, where she worked with Dr. Stephen Heinrichs on the effects of baclofen on cue-induced cocaine relapse.

Michele is the current leader of Team Cocaine, and is characterizing how both cocaine self-administration and cocaine withdrawal change adult neurogenesis in the two neurogenic regions of the adult brain: the subgranular zone (SGZ) of the adult hippocampus, and the subventricular zone (SVZ), which generates neurons that migrate to the olfactory bulb. In the future she plans to test the function of adult neurogenesis in addiction by using the lab's transgenic mice to knockout adult neurogenesis, and to see how this knockout changes both cocaine self-administration or relapse to drug taking, as well as other models of addiction, conditioned place preference (CPP) and conditioned locomotor sensitization.

When Michele is not in lab, she enjoys playing with her dogs Peighton and Sidney, cooking with her husband Tim, and spazzing out on her Nintendo Wii.

Jessica Ables

Jessica, or JAbles, as we call her, joined the lab in September of 2005 after completing her first two years of medical school and passing her Step One board exam. She received her Bachelor's of Science in Chemistry at Southwestern University in Georgetown where she worked with Dr. Kerry Bruns on peptide synthesis.

JAbles is part of Team Prozac, and works with our transgenic mouse lines to examine changes in bHLH transcription factors in collaboration with the Johnson Lab. She is interested in regulation of the bHLH factors that promote neural differentiation in a developmental setting by chronic antidepressants and stress in an adult setting. She plans to use our inducible transgenic to knock-out genes of interest and to ablate the neural progenitor cells to examine the regulation of adult neurogenesis and to examine its similarity to developmental neurogenesis.

When JAbles is not in the lab, she enjoys making a fool of herself in front of the camera. (See photo of the chicken dance.) Jables also enjoys cartoons (Foster's Home for Imaginary Friends is a good one), video games, rebuilding her computer, drawing, and her two crazy cats, Vash and Maniac.

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Nathan DeCarolis

Gradate Student
Laboratory Location: NC6.506
Phone Number: (214)648-1144
Email: nathan.decarolis@utsouthwestern.edu

Nathan aka Nate (Editor's Note: We call him "Flash") DeCarolis joined the Eisch Team in March, 2006, after rotating in the Eisch Lab in the Summer of 2005. For his rotation he examined the impact of cocaine on adult neurogenesis in HDac5 knockout mice. He also studied the distribution of NFkb subunits in the adult dentate gyrus.

While studying drug abuse and stem cells has always been of interest to Nate, this Pennsylvania native originally had much more bucolic dreams: He wanted to be a shepherd. In pursuit of his ideal life, he applied into the Wyoming School of Shepherding in 1998 and was crushed by its rejection (Editor's Note: I take no responsibility for what my lab members choose to write here).

Ultimately, Nate left the Snow Belt for schooling at American University in Washington, DC, where he designed his own major in Neuroscience (hey, if they don't have what you want, get into the kitchen and make it yourself!; Editor's Note: Way to go Nate!). In addition, while under the mentorship of Tony Riley, Nate earned the prestigious Barry M. Goldwater Scholarship. Following graduation, Nate worked at two different biotech companies, Human Genome Sciences and Chesapeake PERL, where he learned a breadth of research skills that paved the way into graduate school.

Since joining the Eisch lab as a member of Team Cocaine, Nate has also pursued some research into neural stem cells in the adult mouse brain. Based on some exciting initial findings, Nate now considers himself a "stem cell ethologist" on the hunt for the elusive neural stem cell in its natural environs of the neurogenic niche.

When not working in the lab (by "working" he really means playing around, making diagrams, and checking e-mail constantly; Editor's Note: this is SO not true - he works really hard), Nate is an up-and-coming member of the Dallas elite (Editor's Note: See next sentence for proof that this sentence is sarcastic). He plays in a community concert band, is active in his church as an altar minister, and plots to take over the world. In addition to hosting lavish parties, Nate also enjoys traveling and cooking. While his dreams of becoming a sheep-tender have dwindled, his new hope is to grow up to be Amy Arguello. Hey, a man can dream big, can't he?

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Laure Farnbauch

Laure has been a research associate in the Eisch lab since August 2004. She's in charge of maintaining our animal colony and conducting the genotyping of our transgenic mice (which has given her the opportunity to learn and perform lots of PCR!). When time allows she participates in some of the lab s research, such as our inducible mouse model and a social stress model of depression. She really enjoys being a member of the Eisch lab and all of it s perks (Great American Hero, Milwaukee Joe s, the Inwood Café, and of course cookies galore!; Editor's Note: I would buy Laure a Ferrari if I could - she is THAT good at what she does... the least she deserves is sandwiches and cookies!).

Now she and her husband are enjoying the warm, sunny climate here in TX, settling into their new house with the new addition of their adorable rat terrier puppy, Sandy! When not in the lab, Laure likes to watch TV (DVRs are a wonderful thing), movies and read horror and fiction (her latest favorite author is Laurel K Hamilton). Oh, and her favorite color is blue! (Editor's Note: ok, I'll start saving for the blue Ferrari...)

None right now. However, applications for postdoctoral positions are welcome. Please contact Dr. Eisch directly.

Lauren Dobbs

Lauren was a real trooper during her time in the Eisch Lab. She worked in Dr. Sherwood Brown's clinical research program, but had a hunch she wanted to pursue basic science research. She volunteered her time many hours between 2005 and 2006 to get her feet wet in the lab, and then successfully parlayed that research experience into acceptance to several Ph.D. programs. Way to go, Lauren! She is currently pursuing her doctorate at the University of Oregon Health Science Center in Portland. Have some salmon for us, Lauren!

Chitra Mandyam, PhD

Chitra joined the group in April 2001. In 2001, Chitra completed her Ph.D. with Kelly Standifer in the Department of Pharmacology and Pharmaceutical Sciences at the University of Houston. The topic of her dissertation was mu and ORL1 opioid receptor cross tolerance in human neuronal cells. In 2005, Chitra completed her postdoctoral work in the Eisch Lab and accepted a position at The Scripps Institute in LaJolla, CA working with a pioneer of the addiction field, George Koob.

Chitra came to the lab to combine her background in opioid receptor pharmacology with adult neurogenesis. She worked on several projects, including characterizing progenitor cells in the mouse subgranular zone with both exogenous (BrdU) and endogenous cell cycle markers; identifying alterations in growth factors in the adult mouse SGZ after chronic morphine; and studying the time course of opiate-induced changes in the adult mouse SGZ after chronic morphine. Chitra was awarded a travel fellowship to present these data at the Route 28 Summit in Neurobiology on stem cells in Frauenchiemsee, Germany, and she is the proud awardee of an NRSA individual postdoctoal award from NIDA. Working with Amelia and Ralph DiLeone (when he was here before he left us to return to Yale - sniff, sniff) Chitra created our inducibile transgenic, the nestinCreERT2 mouse.

Chitra is the proud mom of her little boy, Atulya, born in 2003, and wife of Geri, another brainy Mandyam. Chitra is also not a chocolate fan. You can visit Chitra's home website by clicking here.

Rebekah Norris

Beka joined the lab in August 2001. She graduated from Sul Ross State University in Alpine, TX, in May 2001 and worked for a few months as a Veterinary Nurse. Beka explored the immunohistochemistry of the adult rat hippocampus, and her previous animal experience was invaluable, as she oversaw the breeding of our transgenic mouse models. Beka worked on a time-course analysis of how opiates act to decrease hippocampal neurogenesis, and was an active participant in the cocaine and methylphenidate projects.

Beka left the lab in August 2004 to persue her medical education. She lives in Odessa, TX with her husband Jason, and her two cats, Velcro and Cleo.

Cindi Schwartz

Cindi left us to run a huge EM facility in Colorado to either be with her husband or to be near the mountains (see the TEM on her new lab's website). We are stunned that she finds UC Boulder more interesting than UTSWMC (well, you can't become a member of the Rocky Mountain Rescue here in Dallas).

Eckardt Campos

Eckardt is now a 4th year medical student at UT Southwestern Medical Center. He should excel at neuroanatomy since his drawing skills are incredible. Maybe the next Ramon y Cajal?!

Tzong-Shiue "Tony" Yu

Rotated in Fall 2002. While in the Eisch Lab, Tony examined how self-administration of cocaine alters adult hippocampal neurogenesis. He joined Steve Kernie's Laboratory where he is making great strides in examining regulation of adult neurogenesis.

Denise Ramirez

Rotated in Fall 2003. While rotating with us, Denise examined the impact of cocaine self-administration on adult neurogenesis. She joined David Russell's Laboratory where she is pursuing neurochemical and behavioral analysis of his transgenic mice.

Ozgur Karakazu

Rotated in Winter 2003. During his rotation, Ozgur examined the effect of early postnatal exposure to methylphenidate on adult neurogenesis. He joined Scott Cameron's Laboratory and is enjoying working with worms, even more so since he found a cool egg-laying phenotype.

Deanna Wallace-Black

Rotated in Spring 2004. When Deanna rotated with the Eisch Lab, she examined the time course of morphine-induced alteration of growth factors in adult mice. She joined Eric Nestler's Laboratory, so we still get to see her all the time (yeah!). We are also extremely excited that Deanna has comitted to a postdoctoral fellowship in the laboratory of Mark D'Esposito at UC Berkeley to work on human imaging. What, no more natural behaviors in rats, Deanna? We wonder why?!

Euiseok Kim

Rotated in Spring 2004. During his rotation, Euiseok pioneered the pilot work on cocaine self-administration and resultant changes in nuclear morphology. He joined Jane Johnson's Laboratory, where is he doing cutting-edge research on fate mapping of Mash1 in the developing and adult brain. Fortunately we still see him from time to time as we collaborate often with Euiseok on our shared interests on the role of bHLH transcription factors in the regulation of neurogenesis.

Nora Renthal

Rotated in Summer 2004. When Nora rotated with us, she examined morphine-induced cell death in the hippocampus. She joined the Immunology program for her Ph.D. thesis, and even more amazingly, gave birth to her first child recently: a girl names Amber. Way to go Nora! She is one half of the power MSTP couple of Will and Nora Renthal.

ChiHye Chung

Rotated in Fall 2004. During her rotation, ChiHye performed stereological assessment of hippocampal granule cell layer in a mouse model of Alzheimer's Disease. She joined Ege Kavalali's Laboratory for her doctoral research and is making great strides at becoming an expert neurophysiologist.

Charcacia Sanders

Rotated in Winter 2004. For her rotation, Casey, as we call her, examined beta-amyloid plaque distribution in a mouse model of Alzheimer's Disease with regards to alterations in adult neurogenesis. She joined Dean Smith's Laboratory for her thesis work, and everytime I see her she is even more excited about science.

Anita Autry

Kerstin Ure

Rotated in Winter 2006. For her rotation, Kerstin examined the impact of social defeat stress on adult hippocampal neurogenesis. She joined the laboratory Jenny Hsieh where she is working on the role of NRSF in the regulation of fate choice, and extremely cool project. Amelia is pleased to be able to see her in her role as Chair of Kerstin's thesis committee.

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Colleen Dewey

Rotated in Winter 2006. For her rotation, Colleen explored the localization of estrogen receptors on neural stem cells in the adult rat and mouse hippocampus. She joined the laboratory of Gang Yu where she is doing cool work on the proteosome. We're proud that Colleen is supported by the Psychiatry Department's NIDA Training Grant.

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Alan Lesselyong

Rotated in Winter 2007. Alan spearheaded a collaboration between the Eisch Lab and the Russo-Neustast Lab at Cal State LA on nitric oxide and adult neurogenesis. He joined the laboratory of Carol Tamminga but continues to collaborate with the Eisch Lab in a project exploring the extent and regulation of human hippocampal neurogenesis, so we still get to see Alan around the lab a lot.

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Ryan Simonak

Ryan, an undergraduate at Rice University at the time, worked in the Eisch Laboratory during the summers of 2001 and 2002 as a Stanley Summer Scholar. He played an integral role in collecting preliminary data for the departmental Conte Center grant on Midbrain Structures and Depression and in automating the open field apparatus. Ryan is currently in osteopathic school in Fort Worth - way to go, Ryan!

Jessica Yee

Jessica worked in the Eisch Laboratory during the summers of 2003 and 2004 as a Stanley Summer Scholar. She examined the impact of periadolescent methylphenidate (MPD) administration on adult hippocampal neurogenesis, and earned an authorship for this work. Jess is soon to finish her undergraduate degree at Stanford University and will soon move on to medical school - yeah, Jess!

Lina Seikh

Lina, currently an undergraduate at UT Austin, worked in the Eisch Laboratory during the summer of 2003 as a SURF student. (For more information about the Student Undergraduate Research Forum, or SURF, click here.) She further characterized the effect of cocaine self-administration on the newly born cells of the adult rat hippocampus. At the end of the SURF program, she presented a poster comparing the effects of self- and forced-administration.

Amy Mahan

Amy worked in the Eisch Laboratory during the summer of 2005 as our second SURF student. She characterized the impact of sexual experience on the newly born cells in the adult rat hippocampus. Her poster was, well, a hit at the annual SURF poster session. Amy is now a graduate student in neuroscience at the famed Emory University in Atlanta where she works on voles. We are delighted that we get to see Amy every year at the Annual Meeting of the Society for Neuroscience.

Ricky Markus

Ricky worked in the Eisch Laboratory during the summer of 2005 as a volunteer and then during the summer of 2006 as he was awarded a position on the Summer Training in Drug Abuse Program at UT Southwestern. Over these two summers, Ricky was invaluable in characterizing the five different Nestin-CreER^T2 mouse lines and in determining which ones were good expressers, and in exploring the impact of morphine on vasculature in the adult hippocampus. Ricky is continuing his undergraduate degree at the University of Texas at Austin. We are really happy that Ricky stops by the lab every now and then to say "Hi"!

Emily Hsu

Emily worked in the Eisch Laboratory during the summer of 2004. She characterized the expression of GFP in the hippocampus of the Math1/nGFP mice, and won several awards for this work. Emily is now soaking up life in Cambridge, MA as a Harvard Undergraduate. She is cousins with Monica Lu (see below), who is now a Yalie. Let the rivalry begin!

Sean Yue

Sean, currently a senior TAMS student at the University of North Texas, worked in the Eisch Laboratory during the summer of 2005. (For more information about TAMS, or Texas Advanced Math and Science, click here.) He examined whether female mice have increases in hippocampal proliferation in response to antidepressants (yes, they do). Sean has recently written up the results from his work and submitted a paper to Siemens Westinghouse Competition and we are so psyched that he made it to the semifinals! (For more information about the competition, go here.) Sean is now a productive undergraduate student at Cornell University in Ithaca, NY, although from the pictures he sends us he is getting an awful lot of snowboarding in for a college student!

Santhra Sebastian

Santhra, currently a junior TAMS student at the University of North Texas, worked in the Eisch Laboratory during the summer of 2005 exploring the impact of morphine on GFP positive stem cells in the nestinGFP reporter mouse. Last we heard Santhra was hoping to attend Austin College, a phenomenal liberal arts school here in North Texas.

Monica Lu

Monica worked in the Eisch Laboratory during the summer of 2005. She characterized which subtype of newly born cells in the hippocampus respond to antidepressants, and then spent the next summer doing really cool imaging work at MIT. Monica will graduate from the TAMS program at UNT in May, 2007 after having won almost every award in the book. During the Summer of 2007 she will attend the Weismann Institute for International Research in Israel, a real accomplishment, and will enter Yale University as an undergraduate in the fall. Boola boola, Monica! Go blue!

While Stephanie was a student at Highland Park High School, she worked in the Eisch Laboratory with Diane Lagace on many projects. She became an expert at tissue sectioning, mounting, and staining, and continued to produce excellent work even when she had her foot in a cast (not our fault). Stephanie really was a wonder of self-motivation, setting her own hours and always getting things done smoothly. We appreciate (and miss) you, Stephanie!

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David Pyle

David was a QP-SURF student in the Eisch Lab in the Summer of 2006 (he actually knoew our previous SURF student, Amy Mahan). During his summer with us, David made some extremely interesting discoveries about how Cdk5 in the adult dentate gyrus influences adult neurogenesis. He presented a rockin' poster on his work, and we are even more psyched that David is going to enter UT Southwestern's MSTP program in the Fall of 2007! Hopefully this means we'll see more of his smiling Aggie face around here.

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Lots of Summer, 2007 students! Science moves fast, so we don't know what they will be working on yet in the laboratory when they begin in late May, early June of 2007. However, here are their names, a brief description, and the name of the Eisch Lab Member they will work most with this summer:

Angel Cook: High School student, working with graduate student Amy Arguello

Arathi Ramamurthi: TAMS student, working with graduate student Nate DeCarolis

Amina Igeh: TAMS student, working with MSTP graduate student Jessica Ables

Chandana Ravikumar: TAMS student, working with graduate student Michele Noonan

Alex Thorpe: TAMS student, working with Research Associate Laure Farnbauch

Lurit Bepo: TAMS student, working with graduate student Mike Donovan

Jason Wang: TAMS student, working with postdoctoral fellow Diane Lagace

Greg Wallingford: Undergraduate at University of Notre Dame, working with postdoctoral fellow Diane Lagace

Josh Schonborn: Undergraduate at Oberlin College, working with Amelia Eisch as a fellow in the Summer Training in Drug Abuse. Josh also worked with us in the Summer of 2006, so we are happy to have him back, this time as an S-TDA student!

Dante Martinez: Undergraduate at St. Mary's in San Antonio, working with Amelia Eisch as a SURF student

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Current Members	Past Members	Current Rotation Students	Past Rotation Students	Undergraduate & High School Researchers
Diane Lagace Stephanie Fischer Michael Donovan Gwyn Harburg Amy Arguello Michele Noonan Jessica Ables Nate DeCarolis Laure Farnbauch	Lauren Dobbs Chitra Mandyam Rebekah Norris Cindi Schwartz Eckardt Campos		Tony Yu Denise Ramirez Ozgur Karakazu Deanna Wallace Euiseok Kim Nora Renthal ChiHye Chung Charcacia Sanders Anita Autry Phuong Tran James Sullivan Terina Martinez Stephen Bell Kerstin Ure Colleen Dewey Alan Lesselyong	Ryan Simonak Jessica Yee Lina Seikh Amy Mahan Ricky Markus Emily Hsu Sean Yue Santhra Sebastian Monica Lu David Pyle Josh Schonborn Stephanie Rogan Ricky Markus Greg Wallingford Dante Martinez Jason Wang Arathi Ramamurthi Chandana Ravikumar Lurit Bepo Alex Thorpe Amina Igeh Angel Cook