Request for Funding

Medical Student Research Fellowship for Summer 2004

Mentor: Philip Raskin, M.D. and others
Department: Internal Medicine
Room number: G5.238
Mail Code: 8858
Phone number: 82017 / 72017
E-mail: philip.raskin@utsouthwestern.edu

 

Several Projects

Project I title: Preservation of beta-cell function in type 2 diabetes

Human subjects IRB approved project number (where applicable): 1003-623
Animal subjects IRB approved project number (where applicable): NA

Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)

Brief Description of Project: The project is designed to evaluate beta-cell function longitudinally in newly diagnosed type 2 diabetic patients and compare the decline rate in a group of patients treated with insulin and metformin vs. triple oral antidiabetic regimen.

Students will get an introduction into clinical research. They will learn about recruitment of study volunteers, the conduct of a research protocol, data entry and hopefully, some preliminary data analysis.

Previous Research Activities or Publications with Medical Students:

Summer, 1990 (Grant Dorsey)
Summer, 1993 (Oscar Molina)
Summer, 1994 (Michael Morris, Matthew Myers, and Leland Wilfong)
Summer, 1995 (Jason Brown, Craig Simpson, David Sorley)
Summer, 2000

Project II title: Idiopathic Type 1 Diabetes: Pathogenesis and Progression


Idiopathic Type 1 diabetes, or non-autoimmune Type 1 diabetes is a relatively frequent form of diabetes. It almost always occurs in minority individuals, usually African-American people but also in individuals of Hispanic origin. Patients with this form of diabetes usually present with the classical features of typical Type 1, autoimmune diabetes, i.e., weight loss and ketoacidosis, yet they have the phenotypic appearance of Type 2 diabetes, (older age, obesity, etc). Treatment with insulin is usually required at diagnosis but after a variable period of time many of these individuals can discontinue insulin therapy and be treated with a diet and exercise program and/or oral anti-diabetic medications. The specific aim of this project is the study of the natural history of individuals with idiopathic Type 1 (non-autoimmune) diabetes, to demonstrate that idiopathic Type 1 diabetes is a form of insulin deficient diabetes with progressive beta cell failure that is not due to autoimmune destruction of beta cells and to compare the effects of various treatments on the progression of the disease. The design includes the study of insulin secretion and insulin sensitivity in-patients with idiopathic Type 1 diabetes, with a comparison of their results with individuals with autoimmune Type 1 diabetes and those with Type 2 diabetes. These studies will be carried out in a cross-sectional manner on a group of patients in our database, already identified and classified into one of these groups at least four years after diagnosis. We also plan to identify another cohort of individuals at diagnosis and follow the natural history of the disease over the subsequent four to five years. Finally, a comparison of conventional treatment with insulin or conversion to sulfonylureas in individuals with idiopathic Type 1 diabetes will be done with the plan to study outcomes such as glycemic control, insulin secretion, etc, relative to treatment.
We have had medical students from this program every year. Although very few students "get a paper" from their summer work with us, I do think most of our students enjoy the summer and get a brief introduction into patient based clinical research. Whereas, a two month time period may be enough time to complete a laboratory based project it is never sufficient to complete a clinical one. Students who work with us learn how to identify and recruit patients and to initiate a clinical research project. The other potential added value for students who work with us that in the two months they can learn a lot about diabetes. It is not unusual for our students to attend teaching rounds on the diabetes inservice on a regular basis. This usuallay does not interfer with their other work.

Project III title: "Gender and Ethnic Differences in Lipid Parameters in Diabetes Mellitus" and "Menstrual Status, Hormonal Replacement Therapy and Lipid Levels in Diabetes Mellitus"
Human subjects IRB approved project number (where applicable): NA
Animal subjects IRB approved project number (where applicable): NA
Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)
Retrospective chart review
Brief Description of Project 1:
The first project is a continuation of a retrospective review of medical charts of young diabetic individuals hospitalized at the University Diabetes Treatment Center, at Parkland Health and Hospital System. The purpose of this review is to compare the lipoprotein profile of young (ages 18-45 years) men and women with diabetes and correlate their lipoprotein profile with body weight, gender, duration of diabetes and glycemic control. Also, lipoprotein profile among ethnic groups (non-Hispanic whites, Hispanics and African Americans) will be compared. The ultimate purpose of this review is to confirm or not two previous findings: (1) HDL-C levels were similar between genders and among ethnic groups, and (2) HDL-C levels were indirectly associated with hemoglobin A1c levels in all groups.
The second project is a continuation of a retrospective review of medical charts of premenopausal and postmenopausal women with diabetes hospitalized at the University Diabetes Treatment Center, at Parkland Health and Hospital System. The purpose of this review is to compare the lipoprotein profile of premenopausal diabetic women, postmenopausal diabetic taking hormonal replacement therapy and postmenopausal diabetic women not taking hormonal replacement therapy. The lipoprotein profiles will be correlated with hemoglobin A1c, body weight, and duration of diabetes. The ultimate purpose of this review is to confirm or not two previous major findings: (1) HDL-C levels were higher in postmenopausal women on hormonal replacement therapy, and (2) HDL-C were indirectly associated with hemoglobin A1c levels.

Project IV title: "The effects of intensive insulin therapy on cardiovascular risk factors in Hispanics with Type 2 diabetes mellitus"
Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)
Patient-based

Brief Description of Project IV:
The goal of this five-year awarded project is to provide education, intensive intervention and improve Type 2 diabetes control of Hispanics using insulin, diet and exercise. Several cardiovascular risk factors will be correlated with glycemic control and any change in these factors will be correlated with changes in insulin dosage, glycemic control, anthropometric measurements and peripheral insulin sensitivity. Also, these parameters will be compared between men and women, and any gender similarities or differences established.
The medical student will have the opportunity to interview patients, understand their cultural perspective of diabetes, deliver outpatient diabetes treatment and education, and learn and perform the hyperinsulinemic clamp technique and anthropometric measurements. Since the study is still on its initial phase, baseline characteristics will only be compared.

Project V title: "Potential Causes of Weight Gain with Intensive Insulin and Combination Therapy in Type 2 Diabetes"

Human subjects IRB approved project number (where applicable): 0403-218

Animal subjects IRB approved project number (where applicable): n/a

Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects) Patient-based research.

Brief Description of Project: The study is a prospective analysis of body changes after intensive insulin therapy has been initiated. The main question is: what is responsible for the weight gain that follows intensive glycemic control? The secondary question is: where does this weight goes to?

The subjects have poorly controlled type 2 diabetes. The measurement of body fat is done by underwater weighing. Glycosuria is measured by 24-hr urine collections. Appetite is measured by visual analog scales, while activity is estimated by a 7-day recall. Food diaries are obtained as well. Indirect calorimetry at fasting is performed to estimate resting energy expenditure. MRI of abdomen is performed to measure visceral fat. Blood is drawn as well for HbA1c (3 month average blood sugar), glucose, insulin, c-peptide, leptin, as well as liver and kidney function tests. The tests are repeated at 3 and 6 months.

Previous Research Activities or Publications with Medical Students:
Cristina Montalvo and Rodolfo García worked on this project last summer, but the data collected was insufficient for presentation or publication.

 

Project VI title: Comparison of the Inhibitory Effects of Prolonged Hyperglycemia on Insulin Secretion in Idiopathic Type 1 and Type 2 Diabetes

Human subjects IRB approved project number (where applicable): 50

Animal subjects IRB approved project number (where applicable): NA

Project Type : Patient based

Brief Description of Project: This study compares insulin production between healthy volunteers, type 2 diabetes, and a third entity with classification problems known as atypical ketosis-prone diabetes. Our aim is to understand why this last group of diabetic individuals is prone to develop a hyperglycemic crisis known as Diabetic Ketoacidosis while typical type 2 diabetes is not.

This study involves 3 days of constant hyperglycemia, stimulation of insulin secretion by glucose and by the amino acid arginine, frequent clinical and biochemical monitoring during the hyperglycemic phase, and involvement with computer programs that translate C-peptide values to insulin secretion rates.

Atypical ketosis-prone diabetes has been mostly described in men from African origin, African, Afro-Caribbean, or African-American. Our target population consists of African-American men.

Project VII

Project title: The Effects of Olmesartan Medoxomil, Losartan Potassium, and Atenolol on Insulin Sensitivity in Overweight and Obese Subjects with Hypertension

Human subjects IRB approved project number (where applicable): 012004-038

Animal subjects IRB approved project number (where applicable):

Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects) patient-based


Brief Description of Project: This is an open-label, randomized, 3-arm, parallel-group, multicenter trial designed to assess the effects of olmesartan, losartan, and atenolol on insulin sensitivity in subjects with features of the insulin resistance syndrome, including hypertension and overweight/obesity. We hope to randomize between 6 and 15 patients. There will be a 4-week placebo run-in and a 12-week active treatment period. Visits are scheduled at weeks -4 (screening), -3, -2, -1, 0 (randomization), 2, 4, 8, and 12. The primary efficacy variable is the change in insulin sensitivity, as measured by M value derived from hyperinsulinemic-euglycemic clamp procedures that will be performed at randomization and at the end of the study. We will also perform oral glucose tolerance tests at weeks -4 and 12.


Previous Research Activities or Publications with Medical Students: none




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