Medical Student Research Fellowship for Summer 2008
Mentor: W. Matthew Petroll
Room number: E7.234
Mail Code: 9057
Phone number: 648-7216
Project title: Force generation during corneal wound healing
Human subjects IRB approved project number (where applicable):
Animal subjects IRB approved project number (where applicable):
Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)
Brief Description of Project:
Following injury or laser refractive surgery, corneal keratocytes generate
contractile forces which mediate wound closure and extracellular matrix remodeling.
The goal of this project is to measure the changes in force generation by corneal
keratocytes following treatment with specific growth factors present during
wound healing. Corneal keratocytes will be cultured within 3-D collagen matrices,
and treated with key growth factors present during corneal wound healing (e.g.
TGF-beta, PDGF, CTGF). Cellular force generation will be measured using a culture
force monitor system, in which the collagen matrix is suspended between a fixed
point and a force transducer. The student will assist with cell culture, plating
of cells within the 3-D matrices, and recording and analysis of force data.