Request for Funding
Medical Student Research Fellowship for Summer 2011
All descriptions must contain enough detail to permit an assessment of the problem that is to be addressed and the methodologies that are to be employed. Please be careful to outline the role that the student will play in the project that is described. Please ensure that all relevant approval numbers (IRB, IACUC) are provided.
Mentor: W. Matthew Petroll
Room number: E7.224
Mail Code: 9057
Phone number: 648-7216
Project title: Wide-Field Corneal Imaging using an In Vivo Confocal Microscope
Human subjects IRB approved project number (where applicable):
Animal subjects IACUC approved project number (where applicable): 2010-0230
Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)
Brief Description of Project:
Since the mid 1990s, various forms of confocal microscopy have been used to assess wound healing, toxicity and infection of the human cornea. Our laboratory recently modified a laser scanning confocal microscope, to allow significantly improved imaging of the cornea in vivo. A unique advantage of this microscope is the ability to make on-line 2-D composite images, which dramatically expand the effective field of view. Before applying this technique to patient imaging, we need to optimize the scanning protocol and image analysis procedure for the instrument in an animal model. The corneas of normal New Zealand White rabbits will be scanned by the student using the modified microscope with the goal of establishing these parameters. Briefly, the student will focus the microscope on the anterior layer of corneal keratocytes, and begin on-line composite image acquisition. During the acquisition, the objective will be moved horizontally and vertically so that overlapping images covering a wide area of the cornea can be collected. The student will then evaluate different image analysis approaches for stitching these images together using Image J.
Previous Research Activities or Publications with Medical Students: