Medical Student Research Fellowship for Summer 2012
Mentor: Rahul Banerjee, MD and Chris Chen, PhD
Department: Orthopaedic Surgery
Room number: G8-240
Mail Code: 8870
Phone number: 214-648-2210
E-mail: rahul.banerjee@utsouthwestern.edu
Project title: The Role of G-Protein Coupled Estrogen Receptor 1 in the Fracture Healing of Normal and Oophorectomized Mice
Human subjects IRB approved project number (where applicable):
Animal subjects IRB approved project number (where applicable): 2009-0357
Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)
Animal-based research
Brief Description of Project:
Background: Estrogens play a role in bone growth and remodeling. Previous work from our lab has demonstrated that G-protein coupled estrogen receptor 1 functions as an inhibitory downregulator of bone growth and fracture healing. Past studies have demonstrated that oophorectomy results in an impaired fracture healing response.
Specific Aims: Using an established closed femur fracture model in mice, we will test the hypothesis that deletion of the GPER1 gene will result in an accelerated fracture healing response even in an impaired fracture healing model. The specific aims of the study are: 1) to determine the acute (1 week) and long-term (6 weeks) fracture healing responses of the wild-type (GPER1+/+) and knockout (GPER1-/- deficient) mice and 2) to determine the long-term effect (6 weeks) of GPER1 deficiency in bone repair of oophorectomized mice.
Methods: Wild-type and GPER-1 deficient mice will undergo retrograde femur intramedullary nailing and creation of a femur fracture. A subset of each group will be randomly selected for oophorectomy (performed prior to the fracture). Mice will be evaluated at 1 week and 6 weeks utilizing radiology, histology, biomechanical testing, and PCR to evaluate the fracture healing response.
Note: This project is funded by a grant from the Orthopaedic Trauma Association
Previous Research Activities or Publications with Medical Students:
Presentations: (medical student name is in bold)
“Pelvic CT-Based Graphical Modeling for Safe insertion of Anterior and Posterior
Column Screws” S Banerjee BS, C Sadeghi, MD, A Starr, MD, R Banerjee, MD,
Presented at Texas Orthopaedic Association Annual Meeting, Lost Pines, TX, May 2011
“Pelvic CT-Based Graphical Modeling for Safe insertion of Anterior and Posterior
Column Screws” S Banerjee BS, C Sadeghi, MD, A Starr, MD, R Banerjee, MD, Poster
Presentation, A Day to Highlight Research at Parkland, Dallas, TX, May 2011.
“Pelvic CT-Based Graphical Modeling for Safe insertion of Anterior and Posterior
Column Screws” S Banerjee BS, C Sadeghi, MD, A Starr, MD, R Banerjee, MD, Poster
Presentation, Orthopaedic Trauma Association Annual Meeting, San Antonio, TX, October
2011
“The Role of G-Protein Coupled estrogen Receptor 1 in Fracture Healing,” Sagar B. Patel, Rahul Banerjee, James Phelps, Brigham Au, Orhan Oz, Joseph Borrelli, Christopher Chen, Podium Presentation, 50th Medical Student Research Forum, UT Southwestern Medical Center, Dallas, TX, January 2012
“New Software-Aided Guidance for the Placement of Percutaneous Screws in the Pelvic Acetabular Columns,” Soham Banerjee, C Sadeghi, A Starr, RBanerjee, Poster Presentation, 50th Medical Student Research Forum, UT Southwestern Medical Center, Dallas, TX, January, 2012
“The role of GPER1 in Fracture Healing,” Rahul Banerjee, Sagar B. Patel, James Phelps, Brigham Au, Orhan Oz, Joseph Borrelli, Christopher Chen, Podium Presentation at American Academy of Orthopaedic Surgeons Annual Meeting, San Francisco, CA, February, 2012
Publications: (medical student name is in bold)
Banerjee R, Chao J, Sadeghi C, Taylor R, Nickisch F, Fractures of the calcaneal tuberosity treated with suture fixation through bone tunnels, J Orthop Trauma, 2011 Nov; 25(11):685-90.
Banerjee R, Chao JC, Taylor R, Siddiqui A, Treatment of calcaneal tuberosity fractures, J Am Acad Ortho Surg, in press.
Mentor: Rahul Banerjee, MD
Department: Orthopaedic Surgery
Room number: G8.238
Mail Code: 8870
Phone number: 214-648-2210
E-mail: rahul.banerjee@utsouthwestern.edu
Project title: Pelvic CT-based Graphical Modeling for Safe Insertion of Screws
Human subjects IRB approved project number (where applicable): 082010-113
Animal subjects IRB approved project number (where applicable):
Project Type (patient-based research, animal-based research, or basic research; this characterization is only to permit a general classification for grouping similar types of projects)
Patient-based research
Brief Description of Project:
The purpose of this study was to identify and describe a safe, extra-articular pathway for insertion of a screws for pelvic fracture fixation based on computer modeling derived from CT scans. The medical student, Soham Banerjee, designed the main computer algorithm for the software and he continues to work with me on revising this and applying it to the pelvic CT scans. He will now be a 3rd year medical student so we are looking for someone to continue his work with me on this project. We are currently in the process of writing two papers based on this previous work.
Previous Research Activities or Publications with Medical Students:
Presentations: (medical student name is in bold)
“Pelvic CT-Based Graphical Modeling for Safe insertion of Anterior and Posterior
Column Screws” S Banerjee BS, C Sadeghi, MD, A Starr, MD, R Banerjee, MD,
Presented at Texas Orthopaedic Association Annual Meeting, Lost Pines, TX, May 2011
“Pelvic CT-Based Graphical Modeling for Safe insertion of Anterior and Posterior
Column Screws” S Banerjee BS, C Sadeghi, MD, A Starr, MD, R Banerjee, MD, Poster
Presentation, A Day to Highlight Research at Parkland, Dallas, TX, May 2011.
“Pelvic CT-Based Graphical Modeling for Safe insertion of Anterior and Posterior
Column Screws” S Banerjee BS, C Sadeghi, MD, A Starr, MD, R Banerjee, MD, Poster
Presentation, Orthopaedic Trauma Association Annual Meeting, San Antonio, TX, October
2011
“The Role of G-Protein Coupled estrogen Receptor 1 in Fracture Healing,” Sagar B. Patel, Rahul Banerjee, James Phelps, Brigham Au, Orhan Oz, Joseph Borrelli, Christopher Chen, Podium Presentation, 50th Medical Student Research Forum, UT Southwestern Medical Center, Dallas, TX, January 2012
“New Software-Aided Guidance for the Placement of Percutaneous Screws in the Pelvic Acetabular Columns,” Soham Banerjee, C Sadeghi, A Starr, RBanerjee, Poster Presentation, 50th Medical Student Research Forum, UT Southwestern Medical Center, Dallas, TX, January, 2012
“The role of GPER1 in Fracture Healing,” Rahul Banerjee, Sagar B. Patel, James Phelps, Brigham Au, Orhan Oz, Joseph Borrelli, Christopher Chen, Podium Presentation at American Academy of Orthopaedic Surgeons Annual Meeting, San Francisco, CA, February, 2012
Publications: (medical student name is in bold)
Banerjee R, Chao J, Sadeghi C, Taylor R, Nickisch F, Fractures of the calcaneal tuberosity treated with suture fixation through bone tunnels, J Orthop Trauma, 2011 Nov; 25(11):685-90.
Banerjee R, Chao JC, Taylor R, Siddiqui A, Treatment of calcaneal tuberosity fractures, J Am Acad Ortho Surg, in press.